Composite

Part:BBa_K3781216:Design

Designed by: Nicolas Bayer   Group: iGEM21_TU_Kaiserslautern   (2021-10-15)


L1_sAP_Strep_RBD_Strep8His, MocloMania Composite


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 664
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 664
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 602
    Illegal XhoI site found at 6
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 664
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 664
    Illegal NgoMIV site found at 750
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 774


Design Notes

This composite part was assembled from four basic parts that are part of the MocloMania collection. Due to the highly standardizes set of overhangs that all our basic parts comply with, assembly could be achieved by a simple Moclo ligation. The design of this part was motivated by the aim of verifying the correct adaptation of its underlying sub-parts to the MoClo cloning standard, namely that of the Strep-tag II in B3.


Source

For information on the origin of the genetic sequences present in this composite part, please consult the individual basic part pages.